Coding

Part:BBa_K3389002:Design

Designed by: M. Hammes, H. Sanchez   Group: iGEM20_SUNY_Oneonta   (2020-10-23)


RAD27 Flappase coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 273
    Illegal BglII site found at 666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


Design Notes

This sequence was optimized for expression in Escherichia coli.

Source

The source of this sequence is the genomic sequence from Saccharomyces cerevisiae, the RAD27 gene (GenBank accession number: NM_001179679.1).

References

https://www.sciencedirect.com/science/article/pii/S0092867400817894